Introduction to serial dilution

Decimal numbers may be transformed into scientific notations by shifting the decimal number by the same amount as the exponential number. Multiple dilutions are necessary to reduce the concentration of the sample in multiple ways. The reduction in concentration by using less dilutions is achievable by using large-volume diluting.

This is done by using the dilution instead How satisfied are you with this article? Name Email. Sourav Bio. This article writter by Sourav Bio on April 22, Find out more article Last updated on December 8th, ,. Report Feedback Login Please. Steps of A ten-fold dilution. Steps of Ten-fold serial dilutions. Steps of A two-fold dilution. Steps of Two-fold serial dilutions.

Log Dilutions. Decimal Numbers vs Scientific Notation. Multiple Dilutions. Larger Dilutions. Are you stuck with a problem? Ask Question. Get involved! Remember Me. Register Forgot Password Resend activation code. Please Note: this website requires the use of Javascript for proper operation. Please enable Javascript in order to experience the full capabilities of the application.

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Top 14 books for Industrial Microbiology. Top 15 Books for Food and diary Microbiology. Top 18 books for Environmental Microbiology. Insert the blunt end into a pipette pump, which is a device used to draw liquid into the pipette using suction power. When you are ready to transfer liquid, grasp the pipette pump and remove the pipette from the wrapper. Place the tip of the pipette into the source liquid. Carefully move the wheel of the pipette pump and watch the liquid in the tube; stop when it reaches the mark for the desired volume.

Move the pipette to the destination and dispense the liquid by pulling the trigger, moving the wheel, or pressing on the plunger of the pipette pump. Dispose of the pipette after transfer; they are single-use only. Do not return the pipette to its wrapper; place it in the designated pipette bins.

The wrapper should be thrown away elsewhere. Never use a pipette more than once; if you need to transfer another volume of liquid, always use a fresh pipette. As you can see, the liquid does not disperse completely after transfer. The solution must be mixed by gently inverting the tube or by vortexing.

Mixing the sample is extremely important. The transfer procedure can be repeated as many times as necessary to get the desired number of dilutions. With the proper technique, the dilutions will cover a range of concentrations in discrete, consistent, measurable steps.

Sometimes a solution of bacteria is too concentrated to work with.